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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1980-9-28
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pubmed:databankReference |
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pubmed:abstractText |
The region of mitochondrial DNA (mtDNA) containing the oxi 2 locus has been sequenced in a rho- clone (DS40) derived from the respiratory competent strain D273-10B/A48 of Saccharomyces cerevisiae. The DS40 clone was established to have retained only genetic markers in the oxi 2 locus and to have a segment of mtDNA extending from 18.6 to 24.3 units of the wild type map. The mitochondrial genome of DS40 includes a sequence that has been tentatively identified as the structural gene of Subunit 3 of cytochrome oxidase. The coding sequence is 810 nucleotides long and generates a protein with a molecular weight of 30,340. The amino acid composition of the oxi 2 gene product deduced from the nucleotide sequence is in agreement with the composition of the purified Subunit 3 of yeast cytochrome oxidase. The orientation of the DS40 mtDNA segment relative to wild type mtDNA indicates that the oxi 2 gene is transcribed from the same DNA strand as the oxi 1 and several other mitochondrial genes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
255
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6173-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6248512-Adenosine Triphosphatases,
pubmed-meshheading:6248512-Amino Acids,
pubmed-meshheading:6248512-Base Sequence,
pubmed-meshheading:6248512-DNA, Fungal,
pubmed-meshheading:6248512-DNA, Mitochondrial,
pubmed-meshheading:6248512-DNA Restriction Enzymes,
pubmed-meshheading:6248512-Electron Transport Complex IV,
pubmed-meshheading:6248512-Genes,
pubmed-meshheading:6248512-Mitochondria,
pubmed-meshheading:6248512-Mutation,
pubmed-meshheading:6248512-Nucleotides,
pubmed-meshheading:6248512-Saccharomyces cerevisiae
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pubmed:year |
1980
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pubmed:articleTitle |
Assembly of the mitochondrial membrane system. Sequence of the oxi 2 gene of yeast mitochondrial DNA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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