| pubmed-article:6248422 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6248422 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:6248422 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:6248422 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:6248422 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:6248422 | lifeskim:mentions | umls-concept:C0046803 | lld:lifeskim |
| pubmed-article:6248422 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:6248422 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:6248422 | pubmed:dateCreated | 1980-9-23 | lld:pubmed |
| pubmed-article:6248422 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6248422 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6248422 | pubmed:abstractText | Cordycepin-5'-triphosphate (3'-deoxyadenosine-5'-triphosphate) can be incorporated into the 3'-ends of DNA fragments using terminal deoxynucleotidyl transferase from calf thymus (Bollum, 1974). Because cordycepin-5'-monophosphate lacks a 3'-OH group, only a single residue is incorporated. Furthermore, DNA molecules that contain cordycepin-5'-monophosphate at their 3'-ends become resistant to hydrolysis by exonucleases that require free 3'-OH ends. As an alternative to 5'-end labeling of complementary DNA strands, we have used [32P]cordycepin-5'-triphosphate labeling of 3'-ends to confirm the nucleotide sequence of a HhaI-endonuclease-generated pTU4-plasmid DNA fragment that contains several hot spots for insertions of the transposable genetic element Tn3. 3'-End labeling with [32P] cordycepin-5'-triphosphate has also proved useful in determining the sequence of the pTU4 DNA in the vicinity of a strategically located SstII endonuclease cleavage site in the replication region of the plasmid. | lld:pubmed |
| pubmed-article:6248422 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6248422 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6248422 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6248422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6248422 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6248422 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:6248422 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:6248422 | pubmed:author | pubmed-author:CohenS NSN | lld:pubmed |
| pubmed-article:6248422 | pubmed:author | pubmed-author:TuC PCP | lld:pubmed |
| pubmed-article:6248422 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6248422 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:6248422 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6248422 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6248422 | pubmed:pagination | 177-83 | lld:pubmed |
| pubmed-article:6248422 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:6248422 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:6248422 | pubmed:articleTitle | 3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate. | lld:pubmed |
| pubmed-article:6248422 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6248422 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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