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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1980-9-23
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pubmed:databankReference |
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pubmed:abstractText |
Cordycepin-5'-triphosphate (3'-deoxyadenosine-5'-triphosphate) can be incorporated into the 3'-ends of DNA fragments using terminal deoxynucleotidyl transferase from calf thymus (Bollum, 1974). Because cordycepin-5'-monophosphate lacks a 3'-OH group, only a single residue is incorporated. Furthermore, DNA molecules that contain cordycepin-5'-monophosphate at their 3'-ends become resistant to hydrolysis by exonucleases that require free 3'-OH ends. As an alternative to 5'-end labeling of complementary DNA strands, we have used [32P]cordycepin-5'-triphosphate labeling of 3'-ends to confirm the nucleotide sequence of a HhaI-endonuclease-generated pTU4-plasmid DNA fragment that contains several hot spots for insertions of the transposable genetic element Tn3. 3'-End labeling with [32P] cordycepin-5'-triphosphate has also proved useful in determining the sequence of the pTU4 DNA in the vicinity of a strategically located SstII endonuclease cleavage site in the replication region of the plasmid.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-83
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6248422-Attachment Sites, Microbiological,
pubmed-meshheading:6248422-Base Sequence,
pubmed-meshheading:6248422-DNA, Bacterial,
pubmed-meshheading:6248422-DNA, Circular,
pubmed-meshheading:6248422-DNA Nucleotidyltransferases,
pubmed-meshheading:6248422-DNA Transposable Elements,
pubmed-meshheading:6248422-Deoxyadenine Nucleotides,
pubmed-meshheading:6248422-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6248422-Escherichia coli,
pubmed-meshheading:6248422-Methods,
pubmed-meshheading:6248422-Phosphorus Radioisotopes,
pubmed-meshheading:6248422-Plasmids
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pubmed:year |
1980
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pubmed:articleTitle |
3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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