Linked Life Data

6248399

Source:http://linkedlifedata.com/resource/pubmed/id/6248399

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-9-28
pubmed:abstractText
Prostaglandin (PGE2, PGF2 alpha) production by bovine fasciculo-reticulata adrenocortical cell suspensions was examined using specific radioimmunoassay procedures. No detectable PGs (greater than 50 pg) could be measured in the extract from up to 2 x 10(6) cell incubations after 1 h, with or without the presence of ACTH, although these cells expressed full steroidogenic capabilities under these conditions. The same preparations could produce PGs when supplemented with arachidonic acid but ACTH had no effect on this process. These negative findings could not be explained by analytical artifacts or metabolic transformation. However, an active PG synthetase system was characterized in bovine adrenocortical subcellular preparations. This system converted arachidonate and endogenous substrate(s) to PGE2 as the major product. No thromboxane or prostacyclin pathways were detected even at high enzyme/substrate ratio. Although the microsomal adrenal cortex PG synthetase activity shares many features with those observed in other tissues (Km = 8.3 x 10(-5) M, optimal pH at 8.0, stimulation of PGE2 formation in the presence of glutathione and L-epinphrine), its specific activity was comparatively low (Vmax = 2.5 ng PGE2/min/mg microsomal proteins), which may explain our negative findings using cell suspensions. These findings do not provide evidence to support the hypothesis proposing a role of endogenous PGs in the mechanism of acute ACTH action in the case of bovine adrenal cortex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
249-60
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Prostaglandin synthetase activity in bovine adrenocortical cells and subcellular preparations: effect of ACTH.
pubmed:publicationType
Journal Article