Linked Life Data

6247322

Source:http://linkedlifedata.com/resource/pubmed/id/6247322

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1980-8-15
pubmed:abstractText
Vibrio alginolyticus produces an extracellular collagenase which requires specific induction by collagen or its high-molecular-weight fragments. Peptone also induces collagenase during the late exponential and early stationary growth phases. The peptone inducers have been shown to have a broad molecular weight range between 1,000 and 60,000. The peptone inducers supported slow growth of V. alginolyticus when supplied as the sole nitrogen source in minimal medium. Digestion of the peptone inducers with purified V. alginolyticus collagenase resulted in a decrease in their inducing ability, whereas digestion with trypsin or alpha-chymotrypsin did not. This indicated that induction by the inducers required the presence of collagenase-sensitive bonds. Prolonged digestion of the inducers with collagenase did not completely eliminate the inducing ability of the inducers. The peptone inducers acted as inhibitors of collagenase. A minimal medium induction system has been developed which involves resuspending cells at high density in a medium containing succinate, (NH(4))(2)SO(4), KH(2)PO(4), and the peptone inducer. Cells grown in minimal medium induce earlier than cells grown on peptone, Casamino Acids, or tryptone. Collagenase production was shown to occur for 30 to 60 min in the presence of rifampin at levels which completely inhibit the incorporation of [(3)H]uracil into trichloroacetic acid-precipitable material. Chloramphenicol completely and immediately abolished collagenase production, which together with labeling studies has confirmed that collagenase production involves de novo synthesis of the enzyme. Both glucose and Casamino Acids repressed collagenase production, although synthesis of the enzyme continued for 30 to 60 min after their addition. The repression of collagenase production by glucose and Casamino Acids was more severe than the inhibition of enzyme formation due to addition of rifampin.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-1158851, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-14058277, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-165810, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-165833, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-177067, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-210154, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-334155, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-4152045, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-4200848, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-4352949, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-5040371, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-6314, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-711667, http://linkedlifedata.com/resource/pubmed/commentcorrection/6247322-791074
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
447-54
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Peptone induction and rifampin-insensitive collagenase production by Vibrio alginolyticus.
pubmed:publicationType
Journal Article