|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5762
|
|
pubmed:dateCreated |
1980-7-26
|
|
pubmed:abstractText |
We have used the bacterial plasmid pBR322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. In this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase EcoRI/HindIII insert in BR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.
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|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0028-0836
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
22
|
|
pubmed:volume |
285
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
207-10
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:6246445-Animals,
pubmed-meshheading:6246445-Chickens,
pubmed-meshheading:6246445-Cloning, Molecular,
pubmed-meshheading:6246445-DNA, Recombinant,
pubmed-meshheading:6246445-DNA Restriction Enzymes,
pubmed-meshheading:6246445-Escherichia coli,
pubmed-meshheading:6246445-Genes,
pubmed-meshheading:6246445-L Cells (Cell Line),
pubmed-meshheading:6246445-Mice,
pubmed-meshheading:6246445-Plasmids,
pubmed-meshheading:6246445-Thymidine Kinase,
pubmed-meshheading:6246445-Transformation, Genetic
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|
pubmed:year |
1980
|
|
pubmed:articleTitle |
Isolation of the chicken thymidine kinase gene by plasmid rescue.
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|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
