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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1980-6-16
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pubmed:abstractText |
1. The midpoint potentials of the various iron-sulphur centres in Site I were determined at different pH values by the technique of redox potentiometry. An interesting feature is the pH-dependence of Centre N-2, the highest potential component of the NADH dehydrogenase segment of the respiratory chain. 2. The apparent midpoint potentials of Centre N-2 (NADH dehydrogenase) and S-1 (succinate dehydrogenase) and their pH-dependence was also determined by using the succinate/fumarate couple. Again Centre N-2 is pH-dependent in midpoint potential, and Centre S-1 is not. The results obtained by titrating with the succinate/fumarate couple are in quantitative agreement with those obtained for these centres by redox potentiometry. 3. Oxidation-reduction titrations of iron-sulphur centres with the couple NADH/NAD+ and an analogue APADH/APAD+ in the presence of rotenone gave results substantially different from those obtained by redox potentiometry; these differences may be due to the mechanism of action of NADH dehydrogenase and its specific interaction with NADH. 5. The addition of ATP to an NAD+/NADH-poised system induces an uncoupler-sensitive oxidation of Centre N-4.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-11748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-13905327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-14928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-166670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-179811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-189811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-193495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-199254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-23313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-235450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-23760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-4150420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-4334905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-4335838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-4339790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6245637-793901
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
186
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
111-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6245637-Adenosine Triphosphate,
pubmed-meshheading:6245637-Animals,
pubmed-meshheading:6245637-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:6245637-Electron Transport,
pubmed-meshheading:6245637-Hydrogen-Ion Concentration,
pubmed-meshheading:6245637-Iron-Sulfur Proteins,
pubmed-meshheading:6245637-Membrane Potentials,
pubmed-meshheading:6245637-Metalloproteins,
pubmed-meshheading:6245637-Mitochondria, Heart,
pubmed-meshheading:6245637-NAD,
pubmed-meshheading:6245637-Oxidation-Reduction,
pubmed-meshheading:6245637-Potentiometry,
pubmed-meshheading:6245637-Thermodynamics
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pubmed:year |
1980
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pubmed:articleTitle |
An analysis of some thermodynamic properties of iron-sulphur centres in site I of mitochondria.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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