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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1980-6-25
|
| pubmed:abstractText |
Hepatitis B surface antigen (HBsAg) may be insolubilized onto a controlled pore glass (CPG) matrix by simple adsorption from acidified serum or plasma. The resultant complex has the properties of an HBsAg-specific immunoadsorbent. In this study, CPG-HBsAg complexes have been used to adsorb anti-HBs from standard immune serum globulin. Specific antibodies were eluted with acidic or chaotropic buffers. Recoveries ranging from 29 to 46 per cent, with purifications of approximately 1000-fold were obtained. Two procedures designed to inactivate hepatitis B virus had no effect upon the immunoadsorbent properties of the CPG-HBsAg complex. The possibility of using this simple method to prepare hepatitis B immune globulin is discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0041-1132
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
20
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
212-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6245484-Antibodies, Viral,
pubmed-meshheading:6245484-Glass,
pubmed-meshheading:6245484-Hemagglutination Tests,
pubmed-meshheading:6245484-Hepatitis B Antibodies,
pubmed-meshheading:6245484-Hepatitis B Surface Antigens,
pubmed-meshheading:6245484-Hepatovirus,
pubmed-meshheading:6245484-Humans,
pubmed-meshheading:6245484-Immunoglobulins,
pubmed-meshheading:6245484-Immunosorbent Techniques,
pubmed-meshheading:6245484-Rubella virus,
pubmed-meshheading:6245484-Staphylococcus
|
| pubmed:articleTitle |
Antibody to hepatitis B surface antigen: immunochemical purification from standard immune serum globulin.
|
| pubmed:publicationType |
Journal Article
|