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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1983-12-17
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pubmed:abstractText |
The T cell proliferative response to autologous non-T cells is termed the autologous mixed lymphocyte reaction (AMLR). Recent studies have suggested that the AMLR represents an inducer circuit for the activation of T8+ suppressor/cytotoxic effector cells. Since atopic dermatitis (AD) patients are deficient in T8+ cytolytic T cell function, we investigated the AMLR in AD. When sheep erythrocytes were used to separate T cells from non-T cells, the AMLR was found to be significantly decreased (P less than 0.001) in AD patients (n = 11; delta cpm = 1,550 +/- 393) when compared with normal control subjects (n = 13; delta cpm = 25,819 +/- 4,609). To exclude the possibility that these results were an artifact of the sheep erythrocyte separation, T cells were also separated on a fluorescence-activated cell sorter after treatment of peripheral blood lymphocytes with the OKT3 monoclonal antibody. AD T cells separated by the latter method were also found to have a significantly reduced AMLR response when compared with similarly treated normal T cells. Co-culture studies using cells from AD patients and their HLA identical siblings indicated that the defect resided at the responder T cell level rather than at the stimulator non-T cell level. Co-culture studies revealed no evidence for excessive suppressor cell activity resulting in the decreased AMLR. However, enumeration of T cells reactive with the monoclonal antibody T29, which recognizes a subset of T cells proliferating in the AMLR, demonstrated that AD patients (n = 8; % T29 = 2.5 +/- 0.7) had a significantly decreased (P less than 0.001) number of circulating T29+ T cells when compared with normal controls (n = 8; % T29 = 10.4 +/- 0.8). These studies suggest that a deficiency of T4+ T29+ cells contributes to the deficient AMLR in AD and possibly underlies the abnormalities of T8+ effector cells present in this disease.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-138752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-150602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-152788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-156235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-156242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-156761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-312651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-312901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-4112467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-4166134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-4186206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-4578158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-6213705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-6220063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-6444646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-6447164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-6447724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-6458039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-6459343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-67825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-6980906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6226679-810508
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1482-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6226679-Adolescent,
pubmed-meshheading:6226679-Adult,
pubmed-meshheading:6226679-Antibodies, Monoclonal,
pubmed-meshheading:6226679-Child,
pubmed-meshheading:6226679-Child, Preschool,
pubmed-meshheading:6226679-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:6226679-Dermatitis, Atopic,
pubmed-meshheading:6226679-Female,
pubmed-meshheading:6226679-HLA Antigens,
pubmed-meshheading:6226679-Humans,
pubmed-meshheading:6226679-Immunity, Cellular,
pubmed-meshheading:6226679-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:6226679-Male,
pubmed-meshheading:6226679-T-Lymphocytes, Cytotoxic
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pubmed:year |
1983
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pubmed:articleTitle |
Impairment of the autologous mixed lymphocyte reaction in atopic dermatitis.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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