Linked Life Data

6225783

Source:http://linkedlifedata.com/resource/pubmed/id/6225783

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1983-11-23
pubmed:abstractText
The size of the Ca2+ pump protein in canine cardiac sarcoplasmic reticulum (SR) membranes was determined using target theory analysis of radiation-inactivation data. Samples of cardiac SR were irradiated in the frozen state with increasing doses of high energy electrons from a Van de Graaff accelerator. The loss of Ca2+-dependent ATPase activity and of ATP-dependent, oxalate-facilitated Ca2+ uptake (loading) with increasing irradiation dosage paralleled one another. Also, the loss of staining intensity of the predominant polypeptide (Mr = 110,000) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis correlated with the loss of ATPase and loading activities. The target size by all three measurements varied between 213,000 and 229,000 Da. We conclude that the Ca2+ pump protein in canine cardiac SR is a dimer.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11997-2001
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Determination of the oligomeric structure of the Ca2+ pump protein in canine cardiac sarcoplasmic reticulum membranes using radiation inactivation analysis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't