Linked Life Data

6224484

Source:http://linkedlifedata.com/resource/pubmed/id/6224484

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-9-9
pubmed:abstractText
Possible direct, product/substrate mediated interactions between the phosphate carrier and/or the adenine nucleotide translocator and the mitochondrial H+-ATPase were investigated by tracer flux experiments. For this purpose the specific radioactivity of ATP synthesized was measured and compared with those of intra- and extramitochondrial precursors. Two experimental strategies were applied: 1) preloading of mitochondria with [32P]Pi before starting oxidative phosphorylation of external ADP; 2) simultaneous addition of tracer amounts of [3H]ADP and [32P]Pi to phosphorylating mitochondria under steady-state conditions. The observed participation of the intramitochondrial Pi pool in oxidative phosphorylation excludes a directly functional interaction between Pi carrier and H+-ATPase. At 5 degrees C an at least partial compartmentation of intramitochondrial ADP during oxidative phosphorylation was found, whereas at 25 degrees C a complete equilibrium of intra- and extramitochondrial [32P]Pi and [3H]ADP could be observed, excluding a functionally important compartmentation of these species in the intramitochondrial space under physiological conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0232-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Involvement of intramitochondrial adenine nucleotides and inorganic phosphate in oxidative phosphorylation of extramitochondrially added adenosine-5'-diphosphate.
pubmed:publicationType
Journal Article