|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1985-2-5
|
|
pubmed:abstractText |
Uptake of uridine was studied in isolated intestinal epithelial cells of guinea pig. Uptake was not severely influenced by metabolism. Free uridine was accumulated within cells 13-fold. Uptake was saturable with an apparent Km value of 46 microM and a Vmax of 0.9 nmol/mg protein per min. Uracil inhibited uptake only slightly; adenosine, guanosine and cytosine inhibited strongly. Antimycin A and ouabain inhibited almost 90%. If the extracellular Na+ concentration was decreased to 5 mM, the rate of uptake decreased 6.5-fold. The stimulatory effect of Na+ was related to the transmembraneous Na+-gradient. Cells from jejunum transported about 30% faster than cells from ileum. In conclusion, isolated enterocytes of guinea pig posses an active transport system for uridine.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0006-3002
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
11
|
|
pubmed:volume |
805
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
370-4
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:6210111-Animals,
pubmed-meshheading:6210111-Biological Transport,
pubmed-meshheading:6210111-Carbon Radioisotopes,
pubmed-meshheading:6210111-Dextrans,
pubmed-meshheading:6210111-Epithelium,
pubmed-meshheading:6210111-Guinea Pigs,
pubmed-meshheading:6210111-Intestine, Small,
pubmed-meshheading:6210111-Kinetics,
pubmed-meshheading:6210111-Male,
pubmed-meshheading:6210111-Potassium,
pubmed-meshheading:6210111-Sodium,
pubmed-meshheading:6210111-Tritium,
pubmed-meshheading:6210111-Uridine
|
|
pubmed:year |
1984
|
|
pubmed:articleTitle |
Uridine uptake by isolated intestinal epithelial cells of guinea pig.
|
|
pubmed:publicationType |
Journal Article,
In Vitro
|
