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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1985-1-3
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pubmed:databankReference |
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pubmed:abstractText |
We have sequenced the 480 base pair (bp) repeating unit of the 5S RNA genes of the Dipteran fly Calliphora erythrocephala and compared this sequence to the three known 5S RNA gene sequences from the Dipteran Genus Drosophila (1,2). A striking series of five perfectly conserved homologies identically positioned within the 5' flanks of all four Dipteran 5S RNA coding regions has thus been identified. The spacing (12-13 bp) between all of these homologies is typical of A form rather than B form DNA. Given that the eukaryotic 5S RNA gene specific initiation factor TFIIIA (3) is a DNA unwinding protein (4), a role for these Dipteran 5' flank homologies in initiation site selection on 5S RNA genes transiently unwound for transcription is suggested. One of the Dipteran homology blocks is highly conserved in sequence and position in all but one of the eukaryotic 5S RNA gene sequences known to date (17/18 genes). Its sequence (consensus: TATAAG) and position (average center: -26 bp) are highly reminiscent of the polymerase II gene 'TATA' box (5).
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-14582194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-204911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-264240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-327336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-413625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-416221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-561661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6153931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6160476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6166388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6170932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6251548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6253949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6254757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6262809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6267288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6275361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6275363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6278410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6278416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6299574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6320111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6322117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6356356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6454495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6572947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6728687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6778618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6787600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6803243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6804932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6896875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6924209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-6941292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-7063022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-7145713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-7155897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-7226225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-7357599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-7357604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-7428038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-7438206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-7443522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6209610-810246
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8193-207
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6209610-Animals,
pubmed-meshheading:6209610-Base Composition,
pubmed-meshheading:6209610-Base Sequence,
pubmed-meshheading:6209610-Cloning, Molecular,
pubmed-meshheading:6209610-DNA,
pubmed-meshheading:6209610-DNA, Superhelical,
pubmed-meshheading:6209610-DNA Restriction Enzymes,
pubmed-meshheading:6209610-Diptera,
pubmed-meshheading:6209610-Drosophila,
pubmed-meshheading:6209610-RNA,
pubmed-meshheading:6209610-Species Specificity
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pubmed:year |
1984
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pubmed:articleTitle |
Conserved 5' flank homologies in dipteran 5S RNA genes that would function on 'A' form DNA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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