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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1984-12-28
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pubmed:abstractText |
Two monoclonal antibodies (KH-1 and KH-2) against a transplanted fibrosarcoma (KMT-17) in WKA rats were produced by fusing a mouse myeloma (P3-X63-Ag8.653) with spleen cells from syngeneic rats hyperimmunized with KMT-17. Both antibodies showed complement-dependent cytotoxicity against KMT-17. By absorption of cytotoxicity, KH-1 reacted with homologous tumor, other syngeneic fibrosarcomas (KMT-80 and KMT-75), and lung and kidney from normal rats. However, KH-2 reacted with many kinds of tumors and various normal tissues. Antigen specificity was tested by complement fixation and/or solid-phase radioimmunoassay using glycolipids isolated from KMT-17 cells and authentic glycolipids. KH-1 reacted with globotriglycosyl ceramide which was not detected on KMT-17 cells and in cross-reacted weakly with IV3-alpha-galactosyl-lactoneotetraglycosyl ceramide, one of the major glycolipids of KMT-17. The immune reaction was inhibited by alpha-methyl-galactose. KH-2 reacted with lactosyl ceramide and lactoneotetraglycosyl ceramide. The reaction was more potently inhibited by lactose than by beta-methyl-galactose. Antibodies with similar specificity to either KH-1 or KH-2 were elevated in syngeneic rat sera after serial immunization with viable KMT-17 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0020-7136
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
689-97
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pubmed:dateRevised |
2007-7-24
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pubmed:meshHeading |
pubmed-meshheading:6209227-Animals,
pubmed-meshheading:6209227-Antibodies, Monoclonal,
pubmed-meshheading:6209227-Antibody-Dependent Cell Cytotoxicity,
pubmed-meshheading:6209227-Antigen-Antibody Complex,
pubmed-meshheading:6209227-Antigens, Neoplasm,
pubmed-meshheading:6209227-Carbohydrates,
pubmed-meshheading:6209227-Cerebrosides,
pubmed-meshheading:6209227-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:6209227-Epitopes,
pubmed-meshheading:6209227-Fibrosarcoma,
pubmed-meshheading:6209227-Glycosphingolipids,
pubmed-meshheading:6209227-Hemagglutination Inhibition Tests,
pubmed-meshheading:6209227-Hemagglutination Tests,
pubmed-meshheading:6209227-Liver Neoplasms, Experimental,
pubmed-meshheading:6209227-Lymphoma,
pubmed-meshheading:6209227-Mice,
pubmed-meshheading:6209227-Mice, Inbred Strains,
pubmed-meshheading:6209227-Neoplasm Transplantation,
pubmed-meshheading:6209227-Rats,
pubmed-meshheading:6209227-Rats, Inbred F344,
pubmed-meshheading:6209227-Rats, Inbred Strains,
pubmed-meshheading:6209227-Transplantation, Isogeneic
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pubmed:year |
1984
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pubmed:articleTitle |
Carbohydrates as antigenic determinants of tumor-associated antigens recognized by monoclonal anti-tumor antibodies produced in a syngeneic system.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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