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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1984-11-28
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pubmed:abstractText |
Human T lymphocytes stimulated with phytohaemagglutinin (PHA) or streptokinase-streptodornase (SK-SD) generate an activity which elicits non-cytotoxic histamine release from human basophils. Filtration of the T lymphocyte-derived activity on columns of Sephadex G-100 and Fractogel 55F sequentially revealed one predominant basophil-activating factor of mol. wt. 70,000-90,000, that was designated BAF-T. BAF-T was composed of two acidic proteins of approximate pI 4.4 and 5.2-5.5, as assessed by isoelectric focusing. The distinction of BAF-T from IgE was confirmed by the failure of BAF-T to bind to an anti-IgE affinity column and the capacity of BAF-T to release histamine maximally from basophils desensitized to IgE-dependent stimuli. The inability of BAF-T to release histamine from human lung mast cells and dog cutaneous mastocytoma cells suggests target cell specificity. The source and activity of BAF-T are consistent with a specific contribution of this mediator to human cellular immune and hypersensitivity responses involving T lymphocytes and basophils.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-313840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-4412169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-4641855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-4733239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-5060290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-6172226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-6183340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-6187880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-6195189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-6306109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-6602201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-6979044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-783235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-88479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6208144-88480
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
227-34
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6208144-Basophils,
pubmed-meshheading:6208144-Chromatography, Gel,
pubmed-meshheading:6208144-Growth Substances,
pubmed-meshheading:6208144-Histamine Release,
pubmed-meshheading:6208144-Humans,
pubmed-meshheading:6208144-Lymphocyte Activation,
pubmed-meshheading:6208144-Molecular Weight,
pubmed-meshheading:6208144-Phytohemagglutinins,
pubmed-meshheading:6208144-Streptodornase and Streptokinase,
pubmed-meshheading:6208144-T-Lymphocytes
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pubmed:year |
1984
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pubmed:articleTitle |
A basophil-activating factor from human T lymphocytes.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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