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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1984-11-5
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pubmed:databankReference |
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pubmed:abstractText |
We have prepared and cloned cDNAs derived from poly(A)+ RNA from a human pulmonary carcinoid tumor rich in immunoreactivity to gastrin-releasing peptide, a peptide closely related in structure to amphibian bombesin. Mixtures of synthetic oligodeoxyribonucleotides corresponding to amphibian bombesin were used as hybridization probes to screen a cDNA library prepared from the tumor RNA. Sequencing of the recombinant plasmids shows that human gastrin-releasing peptide (hGRP) mRNA encodes a precursor of 148 amino acids containing a typical signal sequence, hGRP consisting of 27 or 28 amino acids, and a carboxyl-terminal extension peptide. hGRP is flanked at its carboxyl terminus by two basic amino acids, following a glycine used for amidation of the carboxyl-terminal methionine. RNA blot analyses of tumor RNA show a major mRNA of 900 bases and a minor mRNA of 850 bases. Blot hybridization analyses using human genomic DNA are consistent with a single hGRP-encoding gene. The presence of two mRNAs encoding the hGRP precursor protein in the face of a single hGRP gene raises the possibility of alternative processing of the single RNA transcript.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-351419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-383576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-390417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-414220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-4525168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-467325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-4919547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-496973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-496992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-5544731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6133871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6169715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6192430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6194407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6195531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6272398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6276766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6285381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6296591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6339534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6345794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6616473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6669737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6700381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6853532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-6882442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-7029673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-7078353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-7099265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-7099761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-7215543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-7374461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-822353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-823012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-860130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6207529-911786
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5699-703
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6207529-Amino Acid Sequence,
pubmed-meshheading:6207529-Base Sequence,
pubmed-meshheading:6207529-Bombesin,
pubmed-meshheading:6207529-Carcinoid Tumor,
pubmed-meshheading:6207529-Cloning, Molecular,
pubmed-meshheading:6207529-DNA,
pubmed-meshheading:6207529-DNA Restriction Enzymes,
pubmed-meshheading:6207529-Gastrin-Releasing Peptide,
pubmed-meshheading:6207529-Humans,
pubmed-meshheading:6207529-Liver Neoplasms,
pubmed-meshheading:6207529-Nucleic Acid Hybridization,
pubmed-meshheading:6207529-Peptides,
pubmed-meshheading:6207529-Poly A,
pubmed-meshheading:6207529-RNA,
pubmed-meshheading:6207529-RNA, Messenger
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pubmed:year |
1984
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pubmed:articleTitle |
Cloning and characterization of cDNAs encoding human gastrin-releasing peptide.
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pubmed:publicationType |
Journal Article,
Comparative Study
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