Linked Life Data

6207226

Source:http://linkedlifedata.com/resource/pubmed/id/6207226

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1984-11-21
pubmed:abstractText
Murine splenic B lymphocytes were separated into size-dependent subpopulations by using counterflow centrifugation. Spleen cells were rigorously depleted of T lymphocytes to yield a population of cells that were greater than 90% surface immunoglobulin (Ig)-positive and that had a mean cell volume of 136.6 +/- 3.3 microns. From this population, five fractions of cells were obtained with mean cell volumes that ranged from 115.8 +/- 3.7 microns in fraction 1 to 168.0 +/- 6 microns in fraction 5. The cells in these five subpopulations were characterized by analysis on a fluorescence-activated cell sorter after staining with acridine orange to evaluate RNA and DNA content, and with fluorescein-conjugated anti-mu, anti-delta, and anti-Ia antibodies to evaluate their surface membrane phenotypes. DNA analysis revealed that virtually all of the cells in fractions 1 to 4 had 2 N DNA. Between 7 and 21% of fraction 5 cells were either in S-phase or contained 4 N DNA. In contrast, RNA content increased through the fractions, suggesting a transition from G0 to G1 in the subpopulations with increasing B cell size. As another measure of cell activation seen with increasing cell size, we observed a progressive increase in the expression of surface Ia and a decrease in the expression of surface IgD. In the absence of in vitro stimulation, the larger cells showed significantly higher levels of thymidine incorporation. When polyclonal B cell activators such as LPS or anti-Ig antibody were added, peak proliferative responses were similar in all of the fractions, but the time necessary to achieve a maximal response was shorter for the larger-sized cell subpopulations than it was for the smaller-sized cell subpopulations. Unprimed, size-dependent B lymphocyte subpopulations exhibited spontaneous or "background" antibody formation that occurred primarily in the subpopulations containing the largest cells. T cell factors present in EL4 supernatant enhanced the efficiency of in vitro differentiation of these same subpopulations. When cultured in the absence of T cell help, the thymus-independent type 1 (TI-1) antigen TNP-Brucella abortus (TNP-BA) or the thymus-independent type 2 (TI-2) antigen TNP-Ficoll induced the largest anti-TNP plaque-forming cell (PFC) responses in the fractions containing intermediate-sized cells, suggesting that in vitro, antigen-specific responses came primarily from B cells that have been influenced in vivo to leave their small resting state. The subpopulations containing the smallest size B cells required the presence of both a TI antigen and EL4 supernatant for efficient differentiation.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
133
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2333-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:6207226-Animals, pubmed-meshheading:6207226-Antibody-Producing Cells, pubmed-meshheading:6207226-Antigens, Surface, pubmed-meshheading:6207226-Antigens, T-Independent, pubmed-meshheading:6207226-B-Lymphocytes, pubmed-meshheading:6207226-Brucella abortus, pubmed-meshheading:6207226-Cell Cycle, pubmed-meshheading:6207226-Cell Separation, pubmed-meshheading:6207226-Ficoll, pubmed-meshheading:6207226-Hemolytic Plaque Technique, pubmed-meshheading:6207226-Interleukin-2, pubmed-meshheading:6207226-Lymphocyte Activation, pubmed-meshheading:6207226-Macrophages, pubmed-meshheading:6207226-Mice, pubmed-meshheading:6207226-Mice, Inbred CBA, pubmed-meshheading:6207226-Nitrobenzenes, pubmed-meshheading:6207226-Phenotype, pubmed-meshheading:6207226-Polysaccharides, pubmed-meshheading:6207226-Trinitrobenzenes, pubmed-meshheading:6207226-alpha-Macroglobulins
pubmed:year
1984
pubmed:articleTitle
Size-dependent B lymphocyte subpopulations: relationship of cell volume to surface phenotype, cell cycle, proliferative response, and requirements for antibody production to TNP-Ficoll and TNP-BA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.