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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1984-10-2
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pubmed:abstractText |
We have shown that cytotoxic T lymphocytes (CTL) raised in H-2d mice use H-2Ld but not H-2Dd or H-2Kd antigens as restricting elements in lymphocytic choriomeningitis virus (LCMV) and vesicular stomatis virus (VSV) infections. To localize the regions of H-2Ld protein recognized by CTL, we constructed a recombinant H-2Ld/Dd gene encoding a hybrid antigen with alpha 1 and alpha 2 external domains of H-2Ld and alpha 3, transmembrane and cytoplasmic domains of H-2Dd. The recombinant gene was transfected into mouse cells and the hybrid molecules were characterized serologically, biochemically and functionally. In all assays, H-2Ld/Dd molecules were recognized by LCMV-and VSV-specific H-2Ld-restricted CTL in a manner similar to that of wild-type H-2Ld antigens. Analogous results were obtained with alloreactive CTL. Hybrid antigens containing the alpha 3 domain of H-2Ld fused to alpha 1 and alpha 2 domains of a Qa-2,3 region-encoded antigen were not used as restricting elements by LCMV-specific CTL. These results suggest that H-2Ld-restricted CTL directed against LCMV and VSV recognize determinants controlled by the alpha 1 and/or alpha 2 domains of the H-2Ld molecule.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0093-7711
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
141-54
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:6205987-Animals,
pubmed-meshheading:6205987-Antigen-Antibody Complex,
pubmed-meshheading:6205987-Cloning, Molecular,
pubmed-meshheading:6205987-Cytotoxicity, Immunologic,
pubmed-meshheading:6205987-DNA, Recombinant,
pubmed-meshheading:6205987-DNA Restriction Enzymes,
pubmed-meshheading:6205987-Epitopes,
pubmed-meshheading:6205987-Genes,
pubmed-meshheading:6205987-H-2 Antigens,
pubmed-meshheading:6205987-L Cells (Cell Line),
pubmed-meshheading:6205987-Lymphocytic choriomeningitis virus,
pubmed-meshheading:6205987-Mice,
pubmed-meshheading:6205987-Mice, Inbred BALB C,
pubmed-meshheading:6205987-Plasmids,
pubmed-meshheading:6205987-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:6205987-Thymidine Kinase,
pubmed-meshheading:6205987-Transfection,
pubmed-meshheading:6205987-Vesicular stomatitis Indiana virus
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pubmed:year |
1984
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pubmed:articleTitle |
Cytotoxic T lymphocytes recognize determinants on the BALB/c-H-2Ld molecule controlled by alpha 1 and alpha 2 but not alpha 3 external domains.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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