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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1984-9-11
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pubmed:abstractText |
This report describes a procedure by which high performance liquid chromatographic (HPLC) techniques are used to obtain homogeneous S-antigen. Conventionally prepared S-antigen was purified further by fractionation on the anion exchange Mono-Q column followed by gel filtration on a TSK-3000 column. This procedure produced an S-antigen preparation, which appeared homogeneous by gel electrophoresis using the very sensitive silver staining method. The purified material retained its immunochemical and uveitogenic activity. The availability of homogeneous S-antigen will facilitate studies aimed at elucidating, at the molecular level, the mechanism by which S-antigen induces uveitis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0146-0404
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
977-80
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:6204954-Animals,
pubmed-meshheading:6204954-Antigens,
pubmed-meshheading:6204954-Arrestin,
pubmed-meshheading:6204954-Cattle,
pubmed-meshheading:6204954-Chromatography, High Pressure Liquid,
pubmed-meshheading:6204954-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6204954-Retina,
pubmed-meshheading:6204954-Silver,
pubmed-meshheading:6204954-Staining and Labeling
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pubmed:year |
1984
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pubmed:articleTitle |
Purification of retinal S-antigen to homogeneity by the criterion of gel electrophoresis silver staining.
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pubmed:publicationType |
Journal Article
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