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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1984-9-18
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pubmed:abstractText |
Thirty six cloned hybridomas have been isolated which produce monoclonal antibodies directed against simian virus 40 (SV40) large T tumour antigen. They have been shown to recognize at least six different epitopes along the T antigen polypeptide according to their reaction with the various truncated forms of T antigen expressed by adenovirus-SV 40 hybrid viruses. Sixteen antibodies cross-react with cells infected by the closely related human BK virus. Only two antibodies, PAb1604 and PAb1614, directed against different epitopes of the SV40 T antigen, cross-react with polyoma large T tumour antigen which has a more limited amino acid sequence homology. This cross-reaction is rarely seen with polyclonal antibodies. Monoclonal antibody PAb1620 gave nuclear immunofluorescence only with murine cells transformed by SV40 and was found to react with a complex of T-antigen and 53 000-dalton host-coded protein. All the monoclonal antibodies react with nuclear T antigen and all but four antibodies stained the surface of SV40-transformed cells. These were four of the five antibodies directed against the central third of the T antigen. Thus the monoclonal antibodies show that cell surface T antigen differs from nuclear T antigen, either in accessibility or structure.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-191469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-224215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-225042,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-225566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-227604,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-5037438,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-6157837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-6159482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-6159551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-6163870,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-6253155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-6254066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-6255189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-6258291,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6204863-79181
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1485-91
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6204863-Animals,
pubmed-meshheading:6204863-Antibodies, Monoclonal,
pubmed-meshheading:6204863-Antibody Specificity,
pubmed-meshheading:6204863-Antigens, Polyomavirus Transforming,
pubmed-meshheading:6204863-Antigens, Viral, Tumor,
pubmed-meshheading:6204863-Cell Line,
pubmed-meshheading:6204863-Cell Membrane,
pubmed-meshheading:6204863-Cell Transformation, Viral,
pubmed-meshheading:6204863-Cross Reactions,
pubmed-meshheading:6204863-Epitopes,
pubmed-meshheading:6204863-Mice,
pubmed-meshheading:6204863-Molecular Weight,
pubmed-meshheading:6204863-Papillomaviridae,
pubmed-meshheading:6204863-Polyomaviridae,
pubmed-meshheading:6204863-Simian virus 40,
pubmed-meshheading:6204863-Viral Proteins
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pubmed:year |
1984
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pubmed:articleTitle |
Monoclonal antibodies against simian virus 40 nuclear large T tumour antigen: epitope mapping, papova virus cross-reaction and cell surface staining.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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