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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1984-8-8
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pubmed:abstractText |
The thin layer chromatogram binding assay was used to study the reaction of several natural-monoclonal autoantibodies which recognize sialic acid-dependent antigens of human erythrocytes. Immunostaining of gangliosides derived from human and bovine erythrocytes was achieved with four autoantibodies designated anti-Pr2, anti-Gd, Sa and Fl, each of which has a different haemagglutination pattern with untreated and proteinase-treated erythrocytes and with cells of I and i antigen types. From the chromatogram binding patterns of anti-Pr2 with gangliosides of the neolacto and the ganglio series, it is deduced that this antibody reacts best with N-acetylneuraminic acid when it is alpha 2-3- or alpha 2-6-linked to a terminal Gal(beta 1-4)Glc/GlcNAc GlcNAc sequence and to a lesser extent when it is alpha 2-3-linked to a terminal Gal(beta 1-3)GalNAc sequence or to an internal galactose and when it is alpha 2-8-linked to another, internal N-acetylneuraminic acid residue. The other three antibodies differ from anti-Pr2 in their lack of reaction with glycolipids of the ganglio series. They react with the NeuAc(alpha 2-3)Gal(beta 1-4)Glc/GlcNAc sequence as found in GM3 and in glycolipids of the neolacto series, but show a preference for the latter, longer sequences. Thus all four antibodies react with sialylated oligosaccharides containing i type (linear) and I type (branched) neolacto backbones. Fl antibody differs from the other three in its stronger reaction with branched neolacto sequences in accordance with its stronger agglutination of erythrocytes of I rather than i type. The four antibodies show a specificity for N-acetyl- rather than N-glycolyl-neuraminic acid.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204642-100869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204642-107170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204642-12033406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204642-1251575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204642-13428781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204642-14028302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204642-14066623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204642-14097352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6204642-184827,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
219
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
865-74
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6204642-Animals,
pubmed-meshheading:6204642-Antibodies, Monoclonal,
pubmed-meshheading:6204642-Antigens,
pubmed-meshheading:6204642-Autoantibodies,
pubmed-meshheading:6204642-Brain,
pubmed-meshheading:6204642-Cattle,
pubmed-meshheading:6204642-Chromatography, Thin Layer,
pubmed-meshheading:6204642-Epitopes,
pubmed-meshheading:6204642-Erythrocytes,
pubmed-meshheading:6204642-Gangliosides,
pubmed-meshheading:6204642-Glycolipids,
pubmed-meshheading:6204642-Hemagglutination,
pubmed-meshheading:6204642-Humans,
pubmed-meshheading:6204642-N-Acetylneuraminic Acid,
pubmed-meshheading:6204642-Sialic Acids
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pubmed:year |
1984
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pubmed:articleTitle |
The reactivities of human erythrocyte autoantibodies anti-Pr2, anti-Gd, Fl and Sa with gangliosides in a chromatogram binding assay.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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