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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
|
| pubmed:dateCreated |
1984-8-20
|
| pubmed:abstractText |
We have analyzed the requirements for human U1 RNA transcription catalyzed by RNA polymerase II. In Xenopus laevis oocytes, a human U1 RNA gene with only 231 and 35 nucleotides of the 5' and 3' flanking regions, respectively (Lund, E. and Dahlberg, J. E. (1984) J. Biol. Chem. 259, 2013-2021), is able to support accumulation of human U1 RNA. We show that the point in the template corresponding to the 5' end of U1 RNA is a site of transcription initiation. That result rules out the possibility that the 5' end of U1 RNA is generated by cleavage and capping of a precursor RNA. The accumulation of correctly initiated human U1 RNA transcripts requires at least two essential upstream elements. The region between positions -231 and -203 is indispensable for transcription both in oocytes and in vitro. The other region, between positions -105 and -6, fixes the location of the 5' ends of the U1 RNA transcripts in oocytes while not altering the overall level of transcription. This latter region contains a sequence located around position -50, which we propose serves as the analog of the T-A-T-A sequence in U1 and U2 RNA genes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
259
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
8345-52
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6203910-Animals,
pubmed-meshheading:6203910-Base Sequence,
pubmed-meshheading:6203910-DNA Restriction Enzymes,
pubmed-meshheading:6203910-Female,
pubmed-meshheading:6203910-Genes,
pubmed-meshheading:6203910-Humans,
pubmed-meshheading:6203910-Mice,
pubmed-meshheading:6203910-Mutation,
pubmed-meshheading:6203910-Nucleic Acid Hybridization,
pubmed-meshheading:6203910-Oocytes,
pubmed-meshheading:6203910-Operon,
pubmed-meshheading:6203910-RNA,
pubmed-meshheading:6203910-RNA, Small Nuclear,
pubmed-meshheading:6203910-RNA Polymerase II,
pubmed-meshheading:6203910-Rats,
pubmed-meshheading:6203910-Species Specificity,
pubmed-meshheading:6203910-Templates, Genetic,
pubmed-meshheading:6203910-Transcription, Genetic,
pubmed-meshheading:6203910-Xenopus
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|