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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1984-8-22
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pubmed:abstractText |
Kinetics of pinocytosis of FITC-Dextran (mw 70 000) was analysed in mouse L-cells using flow cytometry with a fluorescence-activated cell sorter (FACS). In each experiment information on 2 X 10(4) individual, living cells was obtained. The population of L-cells thus analysed was shown to be rather homogeneous with respect to cell size, and the peak of the FITC-fluorescence curve--a histogram of the accumulated signals from L-cells exposed to FITC-Dextran--was shown to be representative of the average size of the L-cells. It was found that the cellular accumulation of FITC-Dextran was proportional to the tracer. However, the rate of accumulation decreased with increasing time of incubation (up to 3 h). When cells were pulse-labeled for 10 min at 37 degrees C, rinsed carefully with ice-cold PBS, and reincubated at 37 degrees C for various periods of time, about 50% of the initial cellular FITC-fluorescence disappeared within approximately 15 min of reincubation, whereafter no measurable decrease in cellular fluorescence was seen. In addition, a rapid increase of intact FITC-Dextran molecules in the reincubation medium occurred within the first 15 to 30 min of reincubation, whereafter no further increase took place. Thus, a large portion of previously endocytosed FITC-Dextran becomes rapidly exocytosed, while the rest becomes sequestered within a compartment from where little or no exocytosis occurs. The deviation from linearity in accumulation related to time, and the distinct biphasic kinetics of exocytosis are taken to support a generalized two-compartment model for endocytosis and membrane recycling, the two compartments being endocytic vacuoles (endosomes) and (secondary) lysosomes, respectively.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Dextrans,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/fluorescein isothiocyanate dextran
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0171-9335
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
96-102
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:6203751-Animals,
pubmed-meshheading:6203751-Antigens,
pubmed-meshheading:6203751-Dextrans,
pubmed-meshheading:6203751-Flow Cytometry,
pubmed-meshheading:6203751-Fluorescein-5-isothiocyanate,
pubmed-meshheading:6203751-Fluoresceins,
pubmed-meshheading:6203751-Kinetics,
pubmed-meshheading:6203751-L Cells (Cell Line),
pubmed-meshheading:6203751-Mice,
pubmed-meshheading:6203751-Models, Biological,
pubmed-meshheading:6203751-Pinocytosis
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pubmed:year |
1984
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pubmed:articleTitle |
Kinetics of pinocytosis studied by flow cytometry.
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pubmed:publicationType |
Journal Article
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