Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1984-7-2
|
pubmed:abstractText |
A microplate immune-enzymatic method was developed for detecting serum antiplatelet antibodies. The method involves the use of antigen-coated platelets and alkaline phosphatase-labeled antihuman immunoglobulin. Linear correlation was obtained between the titer of platelet antibodies and substrate conversion. Twenty-four patients with immune thrombocytopenia and 40 normal controls were studied. Eighteen patients and one control were positive. Therefore, sensitivity and specificity were 75% and 97% respectively. ELISA also was found to be more sensitive than the indirect antiglobulin consumption assay (ACA) and appears to be a practical and easy method for routine evaluation of antiplatelet antibodies.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:issn |
0323-4347
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
111
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
66-71
|
pubmed:dateRevised |
2011-11-17
|
pubmed:meshHeading |
pubmed-meshheading:6202601-Binding Sites, Antibody,
pubmed-meshheading:6202601-Blood Platelets,
pubmed-meshheading:6202601-Coombs' Test,
pubmed-meshheading:6202601-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:6202601-Humans,
pubmed-meshheading:6202601-Immunoenzyme Techniques,
pubmed-meshheading:6202601-Isoantibodies,
pubmed-meshheading:6202601-Purpura, Thrombocytopenic
|
pubmed:year |
1984
|
pubmed:articleTitle |
A microplate enzyme-linked immunospecific assay (ELISA) detecting unbound anti-platelet antibodies.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|