Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1984-5-30
pubmed:abstractText
Intermediate filaments (IF) isolated from a variety of cultured cells, conventionally described as fibroblasts, are composed predominantely of proteins of molecular weights of 54,000 and/or 55,000. Less than 15% of the protein found in native IF preparations from these cells is composed of three to four polypeptides of molecular weights 60,000-70,000. We have investigated some biochemical and immunological properties of these proteins isolated from BHK-21 and mouse 3T3 cells. They are capable of forming paracrystals that exhibit a light/dark banding pattern when negatively stained with uranyl acetate. The dark bands are composed of longitudinally aligned approximately 2-nm-diam filaments. The center-to-center spacing between either dark or light bands is 37-40 nm. These dimensions are consistent with the secondary structure of IF polypeptides and suggest that the dark bands represent lateral alignment of alpha-helical coiled-coil domains. Immunoblotting, secondary structure, as well as amino acid composition data indicate that the 60,000-70,000-mol-wt paracrystal polypeptides are similar to keratin. Thus, polypeptides with biochemical and immunological properties of epidermal keratin are present in cells normally considered to be fibroblasts.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-1018318, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-102651, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-106897, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-1191265, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-282627, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-291047, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-293716, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-357009, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-361424, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-368806, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-434458, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-447700, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-4680711, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-566763, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-6183000, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-6186381, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-6188955, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-6204640, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-6756644, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-682195, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-6889923, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-6891619, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-6943573, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-7028745, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-7188712, http://linkedlifedata.com/resource/pubmed/commentcorrection/6201488-7201933
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1231-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Isolation and characterization of keratin-like proteins from cultured cells with fibroblastic morphology.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.