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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1984-6-13
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pubmed:abstractText |
The stability of mRNA for the delta-lysin of Staphylococcus aureus was determined by measuring the residual lysin synthesis after inhibition of DNA-dependent RNA polymerase activity with rifampin. At the late logarithmic-early stationary phase of growth the delta-lysin mRNA was very stable, with a half-life of ca. 20 min. Total cellular RNA was extracted from S. aureus and translated with a modified Escherichia coli S-30 system; delta-lysin was identified amongst the translation products by immunoprecipitation and immunoabsorption. The delta-lysin synthesized in vitro was of a size similar to mature delta-lysin and did not require a signal sequence for secretion from the cell.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-1152477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-12635275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-13267987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-13967637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-14479932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-161608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-16250348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-16557995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-16742449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-272676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-322142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-323853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-332169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-355632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-368581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-378936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-39469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-4179831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-4567470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-471058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-4858491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-4879400,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-5017913,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-7078217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-7398877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-811672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-874452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6201445-902851
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
434-8
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:6201445-Bacterial Proteins,
pubmed-meshheading:6201445-Cell-Free System,
pubmed-meshheading:6201445-DNA-Directed RNA Polymerases,
pubmed-meshheading:6201445-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6201445-Escherichia coli,
pubmed-meshheading:6201445-Hemolysin Proteins,
pubmed-meshheading:6201445-Kinetics,
pubmed-meshheading:6201445-Protein Biosynthesis,
pubmed-meshheading:6201445-RNA, Bacterial,
pubmed-meshheading:6201445-RNA, Messenger,
pubmed-meshheading:6201445-Rifampin,
pubmed-meshheading:6201445-Staphylococcus aureus
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pubmed:year |
1984
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pubmed:articleTitle |
In vitro synthesis of the delta-lysin of Staphylococcus aureus.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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