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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1984-5-11
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pubmed:abstractText |
We examined the effects of the simian virus 40 enhancer sequence on transcription of cloned human alpha- and beta-globin genes shortly after their introduction into cultured mammalian cells. We find that (i) detectable transcription of the beta-globin gene but not the alpha-globin gene requires linkage to the enhancer; (ii) the enhancer increases the amount of beta-globin RNA at least 100-fold but results in only a 5- to 10-fold increase in the amount of alpha-globin RNA; (iii) plasmid replication does not increase the level of beta-globin RNA, regardless of linkage to the enhancer, but does result in an approximately equal to 50-fold increase in the level of alpha-globin RNA; (iv) the enhancer is not required for and does not increase transcription of either gene in 293 cells, an adenovirus 5-transformed human kidney cell line. We also show that an enhancer sequence is not required for activity of the normally enhancer-dependent simian virus 40 early promoter in 293 cells, indicating that these cells contain a trans-acting factor(s) that circumvents the requirement for the enhancer sequence.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-291030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-487437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6188062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6246451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6250722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6259538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6260373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6262784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6269067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6275354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6277501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6277502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6287462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6289326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6290076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6290874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6296253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6297796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6300842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6305503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6305591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6313220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6409417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6409418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6409419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6814763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6856467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-6895866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-7151176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-7151177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6200874-886304
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7428-32
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:6200874-Alpha-Globulins,
pubmed-meshheading:6200874-Base Sequence,
pubmed-meshheading:6200874-Beta-Globulins,
pubmed-meshheading:6200874-Cell Line,
pubmed-meshheading:6200874-Cloning, Molecular,
pubmed-meshheading:6200874-Genes,
pubmed-meshheading:6200874-Genetic Linkage,
pubmed-meshheading:6200874-Genetic Vectors,
pubmed-meshheading:6200874-HeLa Cells,
pubmed-meshheading:6200874-Humans,
pubmed-meshheading:6200874-Plasmids,
pubmed-meshheading:6200874-Simian virus 40,
pubmed-meshheading:6200874-Transcription, Genetic,
pubmed-meshheading:6200874-Transfection
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pubmed:year |
1983
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pubmed:articleTitle |
cis and trans activation of globin gene transcription in transient assays.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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