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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1984-5-11
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pubmed:abstractText |
Human liver mRNA was prepared from a patient homozygous for alpha 1-antitrypsin deficiency (PiZZ) and from a normal subject (PiMM). Both liver RNAs were microinjected into Xenopus oocytes and alpha 1-antitrypsin identified by immunoprecipitation. The normal M variant of alpha 1-antitrypsin is synthesised and secreted by Xenopus oocytes, the abnormal Z protein is not secreted and an intracellular form accumulates in the oocytes. In the presence of tunicamycin an unglycosylated form of M alpha 1-antitrypsin appears in the incubation medium but no corresponding unglycosylated version of the Z protein is secreted.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
168
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
84-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:6200362-Animals,
pubmed-meshheading:6200362-Female,
pubmed-meshheading:6200362-Genetic Variation,
pubmed-meshheading:6200362-Humans,
pubmed-meshheading:6200362-Liver,
pubmed-meshheading:6200362-Oocytes,
pubmed-meshheading:6200362-Poly A,
pubmed-meshheading:6200362-Protein Biosynthesis,
pubmed-meshheading:6200362-RNA,
pubmed-meshheading:6200362-RNA, Messenger,
pubmed-meshheading:6200362-Tunicamycin,
pubmed-meshheading:6200362-alpha 1-Antitrypsin,
pubmed-meshheading:6200362-alpha 1-Antitrypsin Deficiency
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pubmed:year |
1984
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pubmed:articleTitle |
Xenopus oocytes can synthesise but do not secrete the Z variant of human alpha 1-antitrypsin.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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