Linked Life Data

6200271

Source:http://linkedlifedata.com/resource/pubmed/id/6200271

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-5-11
pubmed:abstractText
When human retinas are cultured in the presence of various phosphodiesterase (PDE) inhibitors or cyclic nucleotide analogues, rod photoreceptors undergo degenerative changes followed by cell death within 8 hours of incubation, whereas cone photoreceptors and other retinal cells are affected minimally. In the present study we found that the PDE inhibitor, isobutylmethylxanthine (IBMX), as well as the dibutyryl analogues of cGMP and cAMP, inhibited protein synthesis in short-term cultures of human retinas under conditions where uptake of exogenously supplied 3H-leucine was not diminished (relative to controls). The results of an autoradiographic analysis suggested that inhibition of protein synthesis by these drugs occurred to a greater extent in rod photoreceptors than in cones or other retinal cells, and that this phenomenon happened prior to the onset of any morphological changes. When retinas incubated in IBMX for 4 hours were returned to control medium for an additional incubation, rods recovered their ability to synthesize proteins and cell viability was maintained. The results of polyacrylamide gel electrophoresis indicated that IBMX caused a general inhibition of retinal protein synthesis rather than affecting the synthesis of specific retinal proteins. These observations are discussed with regard to possible mechanisms underlying rod-specific photoreceptor degeneration.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0271-3683
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
383-92
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Differential sensitivity of protein synthesis in human retina to a phosphodiesterase inhibitor and cyclic nucleotides.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't