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rdf:type |
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|
lifeskim:mentions |
|
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pubmed:issue |
3
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pubmed:dateCreated |
1984-3-30
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|
pubmed:abstractText |
Five monoclonal antibodies to human C9 were produced. Three of the antibodies had affinities from 1.6 to 3.1 X 10(9) M-1 and 2 were of lower affinity, 0.1 to 0.3 X 10(9) M-1. Four distinct antigenic sites on C9 were detected. The lowest affinity antibody was immobilised on Sepharose 4B and used to isolate C9 from human plasma. A 1200-fold purification was obtained with yield of 30-40%. The purified antigen maintained full cytolytic activity.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1759
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pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
25
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|
pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
269-81
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6199425-Animals,
pubmed-meshheading:6199425-Antibodies, Monoclonal,
pubmed-meshheading:6199425-Antibody Affinity,
pubmed-meshheading:6199425-Binding, Competitive,
pubmed-meshheading:6199425-Complement C9,
pubmed-meshheading:6199425-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6199425-Epitopes,
pubmed-meshheading:6199425-Guinea Pigs,
pubmed-meshheading:6199425-Humans,
pubmed-meshheading:6199425-Immunologic Techniques,
pubmed-meshheading:6199425-Isoelectric Focusing,
pubmed-meshheading:6199425-Mice,
pubmed-meshheading:6199425-Mice, Inbred BALB C,
pubmed-meshheading:6199425-Molecular Weight,
pubmed-meshheading:6199425-Rabbits
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pubmed:year |
1983
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pubmed:articleTitle |
Immunoaffinity purification of human complement component C9 using monoclonal antibodies.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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