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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1984-2-15
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pubmed:abstractText |
Two in vitro approaches were used to investigate the priming of strong-stop plus DNA by Rous sarcoma virus. This 340-base DNA species is the first major plus-strand DNA product seen in both infected cells and in endogenous reactions of disrupted virions. In the first approach, we set up a reconstructed system in which strong-stop plus DNA was synthesized by reverse transcriptase from a high-molecular-weight minus-strand viral DNA template. This synthesis was shown to be strictly dependent on the addition of primers to the reaction mixture. The addition of high-molecular-weight RNA from both viral and cellular sources, as well as oligodeoxyguanylate, gave specific synthesis of strong-stop plus DNA, whereas the addition of oligodeoxycytidylate-oligodeoxyadenylate and viral 4S RNA did not. In the second approach, strong-stop plus DNA synthesized in melittin-permeabilized virions was examined on a high-resolution polyacrylamide gel. This DNA was shown to have ca. 11 to 13 ribonucleotides at its 5' end. These results indicate that strong-stop plus DNA is initiated on a preformed RNA primer.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-185393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-205652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-219216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-4344646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-4349185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-4355596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-46925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6163427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6176578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6183006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6245249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6255214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6260966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6260968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6264101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6287459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6292503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6299578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-6313965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-7060125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-73185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6197536-92028
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
200-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6197536-Avian Sarcoma Viruses,
pubmed-meshheading:6197536-DNA, Viral,
pubmed-meshheading:6197536-RNA, Viral,
pubmed-meshheading:6197536-RNA-Directed DNA Polymerase,
pubmed-meshheading:6197536-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:6197536-Transcription, Genetic,
pubmed-meshheading:6197536-Virus Replication
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pubmed:year |
1984
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pubmed:articleTitle |
Initiation of plus-strand DNA synthesis during reverse transcription of an avian retrovirus genome.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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