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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1984-1-7
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pubmed:abstractText |
A lymphoblastoid cell line derived from a patient with fragile X chromosome exhibited fragility only when 5-fluoro-2'-deoxyuridine (FUdR) was added to the culture medium. Addition of methionine with FUdR greatly increased the frequency of fragile X. Addition of 5-azacytidine (an inhibitor of methylation at the level of DNA) or S-adenosylhomocysteine (an inhibitor of the synthesis of the methyl group donor S-adenosylmethionine) reversed the effect of methionine as well as that of FUdR. It is proposed that DNA methylation is in some way involved in the mechanism of fragile X expression.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0002-9297
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1270-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6196967-Azacitidine,
pubmed-meshheading:6196967-Cell Line,
pubmed-meshheading:6196967-Culture Media,
pubmed-meshheading:6196967-DNA,
pubmed-meshheading:6196967-Dose-Response Relationship, Drug,
pubmed-meshheading:6196967-Floxuridine,
pubmed-meshheading:6196967-Fragile X Syndrome,
pubmed-meshheading:6196967-Homocysteine,
pubmed-meshheading:6196967-Humans,
pubmed-meshheading:6196967-Karyotyping,
pubmed-meshheading:6196967-Methionine,
pubmed-meshheading:6196967-Methylation,
pubmed-meshheading:6196967-S-Adenosylhomocysteine,
pubmed-meshheading:6196967-S-Adenosylmethionine,
pubmed-meshheading:6196967-Sex Chromosome Aberrations
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pubmed:year |
1983
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pubmed:articleTitle |
Fragile X expression is decreased by 5-azacytidine and S-adenosylhomocysteine.
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pubmed:publicationType |
Journal Article
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