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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1984-1-7
|
| pubmed:abstractText |
Two groups of cloned insulin-specific T cell hybridomas were derived from the fusion of (BALB/c X A/J)F1 T cells with the BW5147 tumor line. Group I responded only to the immunogen pork insulin and failed to respond to rat insulin, which differs only at amino acids A4 and B3. The second group of T cell hybridomas exhibited a broader pattern of reactivity to heterologous insulins because they could be stimulated with the B chain-identical pork, beef, sheep, and human insulins, as well as by isolated oxidized B chain. These hybridomas, however, displayed a much greater reactivity to pork insulin than to beef insulin or to the oxidized B chain. Comparison of the reactivity profiles of these two groups of hybridomas with the three-dimensional structure of the insulin molecule strongly suggested that group I cells were recognizing an immunogenic moiety composed of residues A4 and/or B3 in association with A chain loop determinants and that group II hybrids recognized a moiety composed of the amino acid sequence of the B chain and the A chain loop. Both groups of hybrids were restricted to the high-responder I-Ad allele but could be distinguished by their pattern of alloreactivity. We were unable to show any effect of a panel of monoclonal anti-insulin antibodies on the induction of interleukin 2 secretion by these hybridomas or to demonstrate the presence of idiotypic determinants on these T hybrids by using a panel of anti-idiotypic reagents raised against the monoclonal anti-insulin antibodies.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Idiotypes,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
131
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2868-74
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:6196405-Animals,
pubmed-meshheading:6196405-Antibodies, Monoclonal,
pubmed-meshheading:6196405-Cattle,
pubmed-meshheading:6196405-Clone Cells,
pubmed-meshheading:6196405-Epitopes,
pubmed-meshheading:6196405-Genes, MHC Class II,
pubmed-meshheading:6196405-Histocompatibility Antigens Class II,
pubmed-meshheading:6196405-Humans,
pubmed-meshheading:6196405-Hybridomas,
pubmed-meshheading:6196405-Immunoglobulin Idiotypes,
pubmed-meshheading:6196405-Insulin,
pubmed-meshheading:6196405-Interleukin-2,
pubmed-meshheading:6196405-Macromolecular Substances,
pubmed-meshheading:6196405-Mice,
pubmed-meshheading:6196405-Mice, Inbred A,
pubmed-meshheading:6196405-Mice, Inbred Strains,
pubmed-meshheading:6196405-Protein Conformation,
pubmed-meshheading:6196405-Rats,
pubmed-meshheading:6196405-Sheep,
pubmed-meshheading:6196405-Species Specificity,
pubmed-meshheading:6196405-Swine,
pubmed-meshheading:6196405-T-Lymphocytes
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Fine specificity of cloned insulin-specific T cell hybridomas: evidence supporting a role for tertiary conformation.
|
| pubmed:publicationType |
Journal Article
|