Linked Life Data

6196081

Source:http://linkedlifedata.com/resource/pubmed/id/6196081

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-1-7
pubmed:abstractText
In vivo electrochemical detection of endogenous neurotransmitters was done in the dorsal raphe nucleus of urethane-anesthetized male Sprague-Dawley rats. Stereotaxically implanted carbon paste electrodes were scanned using a cyclic voltammetry amplifier with semiderivative signal processing over the potential range--0.2 to +0.5 V at the rate of 10 mV/s. Two distinct peaks were observed at +0.15 V (peak 1) and +0.25 V (peak 2), respectively. Peak identification was assessed by comparing the oxidation potential observed in vivo with those observed in in vitro experiments using pure catecholamines, indoleamines and their metabolites as well as ascorbic acid. Further characterization of in vivo peaks was done by observing changes in electrochemical peaks as well as tissue neurotransmitter concentrations after pharmacological manipulations. p-Chlorophenylalanine, m-hydroxybenzylhydrazine (NSD-1015), pargyline, alpha-methyl-p-tyrosine and fusaric acid were administered in an effort to block catecholamine or serotonin synthesis or degradation. Results of these experiments revealed that peak 1 primarily represents extracellular norepinephrine, while peak 2 is primarily produced by extracellular 5-hydroxyindoleacetic acid (5-HIAA).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-62
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
In vivo electrochemical detection of extraneuronal 5-hydroxyindole acetic acid and norepinephrine in the dorsal raphe nucleus of urethane-anesthetized rats.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't