|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0017349,
umls-concept:C0018894,
umls-concept:C0024297,
umls-concept:C0030685,
umls-concept:C0332120,
umls-concept:C0391871,
umls-concept:C0540218,
umls-concept:C0591833,
umls-concept:C0599796,
umls-concept:C0680255,
umls-concept:C1167622,
umls-concept:C1283071,
umls-concept:C1514485,
umls-concept:C1522642,
umls-concept:C1533157,
umls-concept:C1548328,
umls-concept:C1553412,
umls-concept:C1963578
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1983-12-17
|
|
pubmed:abstractText |
Monoclonal antibody GK1.5 recognizes a determinant, designated L3T4a, on the murine T cell surface molecule L3T4. The expression of L3T4a by functional murine T cell clones appears to correlate primarily with class II MHC antigen reactivity rather than with functional phenotype. In previous studies, antigen-specific cytolysis by a cloned class II MHC antigen(I-Ak)-reactive CTL line was found to be blocked entirely by monoclonal antibody (mAb) GK1.5, at a step before the lethal hit. In the present studies, we demonstrate that mAb GK1.5 profoundly blocks antigen-specific proliferation and release of lymphokines by cloned murine class II MHC antigen-reactive helper T lymphocyte (HTL) lines. Analysis of cloned T cell hybridomas, however, suggests that there exists clonal heterogeneity in the degree of inhibition of class II MHC antigen-specific function by mAb GK 1.5. Finally, we present evidence that mAb GK1.5 blocks class II MHC antigen-specific function by blocking class II MHC antigen-specific binding. The data presented here lend considerable support to the concept both that L3T4 and the human Leu-3/T4 molecules are similar and that L3T4 plays a role in class II MHC antigen-reactivity by murine T cells.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0022-1767
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
131
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2178-83
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:6195255-Animals,
pubmed-meshheading:6195255-Antibodies, Monoclonal,
pubmed-meshheading:6195255-Antigen-Antibody Reactions,
pubmed-meshheading:6195255-Binding, Competitive,
pubmed-meshheading:6195255-Binding Sites,
pubmed-meshheading:6195255-Binding Sites, Antibody,
pubmed-meshheading:6195255-Clone Cells,
pubmed-meshheading:6195255-Epitopes,
pubmed-meshheading:6195255-Female,
pubmed-meshheading:6195255-H-2 Antigens,
pubmed-meshheading:6195255-Interleukin-2,
pubmed-meshheading:6195255-Lymphocyte Activation,
pubmed-meshheading:6195255-Lymphokines,
pubmed-meshheading:6195255-Mice,
pubmed-meshheading:6195255-Mice, Inbred C57BL,
pubmed-meshheading:6195255-Mice, Inbred CBA,
pubmed-meshheading:6195255-Ovalbumin,
pubmed-meshheading:6195255-T-Lymphocytes, Helper-Inducer
|
|
pubmed:year |
1983
|
|
pubmed:articleTitle |
Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
