rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
12
|
pubmed:dateCreated |
1983-12-17
|
pubmed:abstractText |
Qualitative and quantitative tests were performed to determine whether the temperature at which dehydration and embedding occur affects the antigenic specificity of tubulin and the protein A-gold (pAg) immunolabeling technique. The analysis indicates that low temperature (-35 degrees C) treatment increased the specificity and density of pAg labeled anti-tubulin antibodies to Leishmania tropica subpellicular microtubules as compared to samples prepared at 0 degrees C or 20 degrees C.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0022-1554
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
31
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1380-4
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:6195215-Acrylic Resins,
pubmed-meshheading:6195215-Colloids,
pubmed-meshheading:6195215-Epitopes,
pubmed-meshheading:6195215-Epoxy Resins,
pubmed-meshheading:6195215-Gold,
pubmed-meshheading:6195215-Histocytochemistry,
pubmed-meshheading:6195215-Histological Techniques,
pubmed-meshheading:6195215-Immunologic Techniques,
pubmed-meshheading:6195215-Leishmania,
pubmed-meshheading:6195215-Microscopy, Electron,
pubmed-meshheading:6195215-Microtubules,
pubmed-meshheading:6195215-Staphylococcal Protein A,
pubmed-meshheading:6195215-Temperature,
pubmed-meshheading:6195215-Tubulin
|
pubmed:year |
1983
|
pubmed:articleTitle |
Dehydration and embedding temperatures affect the antigenic specificity of tubulin and immunolabeling by the protein A-colloidal gold technique.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|