Linked Life Data

6195182

Source:http://linkedlifedata.com/resource/pubmed/id/6195182

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-12-17
pubmed:abstractText
Sets of three Isolator blood culture tubes were seeded with low numbers of 96 strains of 26 bacterial species (fresh and stock clinical isolates). One tube was processed immediately, and the other two were held at 22 and 34 degrees C for 15 h before processing. Organism recovery was 99, 99, and 98%, respectively. Organism numbers increased at both 22 degrees C (60% of strains) and 34 degrees C (79% of strains). Especially notable was that the increases were seen with most strains of Staphylococcus aureus, streptococcal species, Pseudomonas, and all of the Enterobacteriaceae tested. Seven strains, including Streptococcus pneumoniae and Haemophilus influenzae, although viable, were recovered with a decreased number of organisms at each temperature. Acridine orange staining detected organisms in 53% of those Isolator tubes being held and 71% of those demonstrating a numerical increase, after incubation at 34 degrees C. In addition, it was noted that after processing, 48% of the strains that had increased in number while being held at 34 degrees C resulted in visible growth on agar media in 6 h. The results suggest that up to a 15-h delay in processing Isolator tubes may be possible and that acridine orange staining for the rapid detection of positive cultures may be useful in such a circumstance.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
709-12
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Viability of organisms held in the isolator blood culture system for 15 h and their rapid detection by acridine orange staining.
pubmed:publicationType
Journal Article