Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0003316,
umls-concept:C0009013,
umls-concept:C0017262,
umls-concept:C0017349,
umls-concept:C0039194,
umls-concept:C0185117,
umls-concept:C0205245,
umls-concept:C0591833,
umls-concept:C1414948,
umls-concept:C1415082,
umls-concept:C1524084,
umls-concept:C1880022,
umls-concept:C2700225,
umls-concept:C2911684
|
| pubmed:dateCreated |
1983-12-20
|
| pubmed:abstractText |
We describe here the properties of mAb GK1.5, which recognizes a cell surface molecule designated L3T4; the determinant on L3T4 recognized by mAb GK1.5 is designated L3T4a. We present evidence here that: i) the expression of L3T4a by murine T cell clones correlates primarily with class II MHC antigen-reactivity; ii) mAb GK1.5 blocks all class II MHC antigen-specific functions (cytolysis, proliferation, release of lymphokines) by murine class II MHC antigen-reactive T cell clones, although there appears to be clonal heterogeneity in the degree to which these functions are blocked by mAb GK1.5; iii) mAb GK1.5 blocks class II MHC antigen-specific release of IL-2 from cloned T cell hybridomas by blocking class II MHC antigen-specific binding; and iv) L3T4 is very similar to the human Leu3/T4 antigen. The properties of mAb GK1.5 (complement fixation, reactivity with all mouse strains tested, profound blocking of all class II MHC antigen-specific functions by murine T cells, usefulness for FACS analyses, and usefulness for immuno-precipitation/SDS-PAGE analyses) make it suitable for investigating both the role of class II MHC antigen-reactive T cells in various immunological phenomena and the mechanistic basis, at the molecular level, of class II MHC antigen-reactivity by murine T cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0105-2896
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
74
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
29-56
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:6195085-Animals,
pubmed-meshheading:6195085-Antibodies, Monoclonal,
pubmed-meshheading:6195085-Antigen-Antibody Reactions,
pubmed-meshheading:6195085-Antigens, Ly,
pubmed-meshheading:6195085-Binding, Competitive,
pubmed-meshheading:6195085-Cytotoxicity, Immunologic,
pubmed-meshheading:6195085-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6195085-Epitopes,
pubmed-meshheading:6195085-Flow Cytometry,
pubmed-meshheading:6195085-Histocompatibility Antigens,
pubmed-meshheading:6195085-Humans,
pubmed-meshheading:6195085-Hybridomas,
pubmed-meshheading:6195085-Lymphocyte Activation,
pubmed-meshheading:6195085-Mice,
pubmed-meshheading:6195085-Mice, Inbred BALB C,
pubmed-meshheading:6195085-Mice, Inbred C57BL,
pubmed-meshheading:6195085-Mice, Inbred CBA,
pubmed-meshheading:6195085-Rats,
pubmed-meshheading:6195085-T-Lymphocytes
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Review,
Research Support, Non-U.S. Gov't
|