Linked Life Data

6194818

Source:http://linkedlifedata.com/resource/pubmed/id/6194818

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
1983-12-17
pubmed:databankReference
pubmed:abstractText
The administration of glucocorticosteroid hormones to newborn rats interrupts selectively (and reversibly, if the hormone is withdrawn) the hepatic production of alpha 1-fetoprotein (AFP). This results from a decreased concentration of AFP mRNA in the liver [Bélanger, L., Frain, M., Baril, P., Gingras, M.C., Bartkowiak, J., & Sala-Trepat, J.M. (1981) Biochemistry 20, 6665]. We have delineated further the mechanism and time course of this hormonal action in 4-day-old rats treated with dexamethasone (DEX). DNA from a recombinant plasmid containing a 578-bp insert of rat AFP cDNA was used to develop a cell-free nuclear run-off system and directly assess AFP gene transcription activity. Five minutes after DEX injection, AFP gene transcription activity is unchanged, but after 30 min, it drops to 25% that of the control; this correlates with the time required for translocation of DEX receptors to the nucleus. Dose-response curves also show that the degree of AFP gene suppression is closely correlated with the amount of DEX receptor translocated to the nucleus. The nuclear concentration of AFP mRNA, monitored by dot-blot hybridization, decreases to undetectable levels within 48 h, whereas that of albumin mRNA increases slightly, which indicates the selectivity of DEX action. These results show that DEX suppresses AFP gene expression at the transcriptional level and suggest a direct negative action of DEX-receptor complexes on the AFP chromatin transcription unit.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4296-302
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Rapid suppression of alpha 1-fetoprotein gene transcription by dexamethasone in developing rat liver.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't