Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1983-10-21
pubmed:abstractText
Obtaining biologically significant fine detailed information from sections is limited firstly by the unknown distribution of heavy metal stain and secondly by conformational changes induced by dehydration. Only afterwards we have to be concerned with beam induced alterations. By Z-imaging in a STEM, completely unstained material can now become imaged sharply with high contrast. By this the first limitation is eliminated and the second can become explored. For this purpose new resins designed for low temperature embedding might become important. First biological results are presented which illustrate the potential of these techniques: The transmembrane protein of the separate junction has been revealed as such and shown that only the hydrophilic part of the protein is stained by uranyl acetate. This type of staining therefore does not allow the detection of any transmembrane proteins in sections.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0385-0005
pubmed:author
pubmed:issnType
Print
pubmed:volume
7 Suppl
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-42
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Perspectives for achieving improved information by the observation of thin sections in the electron microscope.
pubmed:publicationType
Journal Article