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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1983-10-28
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pubmed:abstractText |
The clonal heterogeneity of nine monoclonal antibodies with absolute specificities for deoxyribonucleic acid (DNA) was analyzed. These monoclonal anti-DNA antibodies were generated in three different fusion experiments using autoimmune (NZB X NZW)F1 mouse spleen cells. Isoelectric focusing analyses demonstrated different isoelectric points within the IgG2a and IgG2b subclasses. Three anti-idiotypic antisera were prepared (one in a rabbit and two in mice) against two monoclonal anti-DNA antibodies. These antisera detected idiotypic determinants uniquely associated with homologous hybridoma anti-DNA antibodies. Two of these idiotypes could be detected at low levels in the sera of (NZB X NZW)F1 mice. Anti-PME77 idiotypic antiserum had no effect in vitro on the total binding capacity of (NZB X NZW)F1 sera. Taken together these results demonstrate that, in (NZB X NZW)F1 mice, the anti-DNA antibody repertoire contains molecules that show similar antigen binding characteristics but are not structurally uniform.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-233900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-4757871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-6161826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-6172515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-6181395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-7045222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-7140814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-7191869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-7198664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-7451968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193527-89171
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6024-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6193527-Animals,
pubmed-meshheading:6193527-Antibodies, Monoclonal,
pubmed-meshheading:6193527-Antigen-Antibody Complex,
pubmed-meshheading:6193527-Autoantibodies,
pubmed-meshheading:6193527-DNA,
pubmed-meshheading:6193527-Epitopes,
pubmed-meshheading:6193527-Hybridomas,
pubmed-meshheading:6193527-Immunoglobulin Idiotypes,
pubmed-meshheading:6193527-Mice,
pubmed-meshheading:6193527-Mice, Inbred Strains,
pubmed-meshheading:6193527-Species Specificity
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pubmed:year |
1983
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pubmed:articleTitle |
Murine monoclonal anti-DNA antibodies with an absolute specificity for DNA have a large amount of idiotypic diversity.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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