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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1983-10-28
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pubmed:abstractText |
The gene for thermostable alpha-amylase from the thermophilic bacterium Bacillus stearothermophilus has been cloned and expressed in Escherichia coli. Each alpha-amylase-producing colony contained at least a 9.7-kilobase-pair (kb) chimeric plasmid composed of the vector pBR322 and a common 5.4-kb HindIII fragment of DNA. B. stearothermophilus contains four plasmids with sizes from 12 kb to over 108 kb. Restriction endonuclease analysis of these naturally occurring plasmids showed they also contain a 5.4-kb HindIII fragment of DNA. Cloning experiments with the four plasmids yielded alpha-amylase-producing E. coli that contained the same 9.7-kb chimeric plasmid. Restriction endonuclease analysis and further recombinant DNA experiments identified a 26-kb plasmid that contains the gene for alpha-amylase. A spontaneous mutant of B. stearothermophilus unable to produce alpha-amylase was missing the 26-kb plasmid but contained a 20-kb plasmid. A 6-kb deletion within the region of the 5.4-kb HindIII fragment yielded the 20-kb plasmid unable to code for alpha-amylase. A nick-translated probe for the alpha-amylase coding region did not hybridize to either plasmid or total cellular DNA from this mutant strain of B. stearothermophilus. These results demonstrate the gene for alpha-amylase is located exclusively on a 26-kb plasmid in B. stearothermophilus with no genetic counterpart present on the chromosome.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-1140401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-1141196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-14861189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-232126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-326413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-327269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-331257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-344136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-344137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-4212116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-4215793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-4336693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-4419109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-4559594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-476554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-4894690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-5416895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-5776281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-6336732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-6774224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-6949000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-7019914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-791074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193526-881736
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5975-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:6193526-Amylases,
pubmed-meshheading:6193526-Cloning, Molecular,
pubmed-meshheading:6193526-DNA, Recombinant,
pubmed-meshheading:6193526-DNA Restriction Enzymes,
pubmed-meshheading:6193526-Drug Stability,
pubmed-meshheading:6193526-Genes,
pubmed-meshheading:6193526-Genes, Bacterial,
pubmed-meshheading:6193526-Geobacillus stearothermophilus,
pubmed-meshheading:6193526-Hot Temperature,
pubmed-meshheading:6193526-Nucleic Acid Hybridization,
pubmed-meshheading:6193526-Plasmids,
pubmed-meshheading:6193526-alpha-Amylases
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pubmed:year |
1983
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pubmed:articleTitle |
Bacillus stearothermophilus contains a plasmid-borne gene for alpha-amylase.
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pubmed:publicationType |
Journal Article
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