|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
17
|
|
pubmed:dateCreated |
1983-10-8
|
|
pubmed:abstractText |
A monospecific antibody produced in vitro by a B-lymphoblastoid cell line transformed with Epstein-Barr virus has been shown to recognize a membrane antigen (OFA-I-1) on human tumors and fetal brain. This study identifies the chemical nature of OFA-I-1. The glycolipid fraction of antigen-rich spent medium of an OFA-I-1-positive melanoma cell line, M14, was extracted by chloroform/methanol/water, 4:8:3 (vol/vol), and was separated into fractions of neutral glycolipids and gangliosides by DEAE-Sephadex followed by base treatment and Bio-sil A column elution. OFA-I-1 antigens were found exclusively in the ganglioside fraction when assayed with monospecific anti-OFA-I-1 by an immune adherence inhibition test. The results obtained from thin-layer chromatography of the antigenic M14 ganglioside and sequential glycosidase digestion suggested that the antigen was a ganglioside GM2, GalNAc beta 1 leads to 4(NeuAc alpha 2 leads to 3)Gal beta 1 leads to 4Glc leads to Cer. These results were further supported by testing various authentic gangliosides and neutral glycolipids for OFA-I-1 antigenicity. Only GM2 showed positive reactivity.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-103458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-13346373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-13436486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-13672998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-14174000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-4270451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-4324310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-4413334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-468792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-5892497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-6175965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-61813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-6268878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-6291057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-6296843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-670832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-6929544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-6938976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-7047999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-7061953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-7130176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-7142214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-7209516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-7353026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-849933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6193515-88539
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
80
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5392-6
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:6193515-Adult,
pubmed-meshheading:6193515-Antigens, Neoplasm,
pubmed-meshheading:6193515-Brain,
pubmed-meshheading:6193515-Carbohydrates,
pubmed-meshheading:6193515-Cell Line,
pubmed-meshheading:6193515-Epitopes,
pubmed-meshheading:6193515-Female,
pubmed-meshheading:6193515-Fetus,
pubmed-meshheading:6193515-G(M2) Ganglioside,
pubmed-meshheading:6193515-Gangliosides,
pubmed-meshheading:6193515-Glycolipids,
pubmed-meshheading:6193515-Humans,
pubmed-meshheading:6193515-Liver,
pubmed-meshheading:6193515-Melanoma,
pubmed-meshheading:6193515-Pregnancy
|
|
pubmed:year |
1983
|
|
pubmed:articleTitle |
Ganglioside GM2 as a human tumor antigen (OFA-I-1).
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
