Linked Life Data

6189836

Source:http://linkedlifedata.com/resource/pubmed/id/6189836

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1983-7-8
pubmed:abstractText
Estrogen regulates the synthesis of the egg yolk precursor protein, vitellogenin, by causing both a 20-60-fold increase in the absolute rate of total nuclear RNA synthesis and a selective increase of at least several thousand fold in the absolute rate of vitellogenin gene transcription. Vitellogenin gene transcription is undetectable in unstimulated and withdrawn Xenopus laevis liver cells and in cultured Xenopus kidney cells allowing us to set a very low upper limit (less than 1 transcript/vitellogenin gene/day) on potential basal rates of vitellogenin gene transcription. The elevated rates of vitellogenin mRNA accumulation previously observed during restimulation of withdrawn liver cells (secondary estrogen stimulation) appear to be due to an increased rate of vitellogenin gene transcription. Both the maximum transcription rate and the rapidity of the early response increase on secondary estrogen stimulation. Relative transcription rates were determined by hybridization of pulse-labeled nuclear RNA to vitellogenin cDNA clones immobilized on nitrocellulose filters. The conversion of relative transcription rates to absolute transcription rates, was facilitated by development of a sensitive high performance liquid chromatography method for quantitation of the specific radioactivity of the cellular UTP pool.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5449-55
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Estrogen regulates the absolute rate of transcription of the Xenopus laevis vitellogenin genes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.