Linked Life Data

6189473

Source:http://linkedlifedata.com/resource/pubmed/id/6189473

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1983-6-10
pubmed:abstractText
Keratoplasty specimens from eight patients with granular corneal dystrophy (GCD) and age-matched control subjects were examined by combinations of immunohistological stains, transmission electron microscopy (TEM), and sodium dodecyl sulfate gel electrophoresis. Fresh frozen sections from corneas with GCD stained positively with antibodies to microfibrillar protein by immunofluorescence. Routine TEM disclosed that the granules had central electron-dense areas partially surrounded by 9- to 10-nm tubular microfibrils. Material eluted from corneas with GCD showed denser peptide bands at 65 and 110 kilo than in normal corneas. Stains were negative for elastin, amyloid, neutral lipids, cholesterol, and glycosaminoglycan. Luxol fast blue MBSN stain was strongly positive in the granules in all cases examined. Immunofluorescent stains were negative with antibodies to plasma fibronectin (cold insoluble globulin), laminin, collagens I to V, basement membrane proteoglycan, tropoelastin, and keratin. In two corneas with GCD an increased lipid content was found in every phospholipid class, although cholesterol content was unchanged. Alterations in the fatty acid profiles of phospholipids were also observed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-9950
pubmed:author
pubmed:issnType
Print
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
802-10
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Microfibrillar protein and phospholipid in granular corneal dystrophy.
pubmed:publicationType
Journal Article