Linked Life Data

6188036

Source:http://linkedlifedata.com/resource/pubmed/id/6188036

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-5-5
pubmed:abstractText
The metabolism and inhibitory activity of 2-beta-D-ribofuranosylthiazole-4-carboxamide (tiazofurin, RTC, NSC 286193) was studied in Chinese hamster ovary (CHO) cells and a series of mutants derived from them. Isotope incorporation experiments indicate dramatic inhibition of DNA and RNA synthesis by tiazofurin but little or no effect on protein synthesis. In CHO cells, tiazofurin is more toxic than ribavirin and also demonstrates a stronger inhibition of guanine nucleotide synthesis. Tiazofurin is phosphorylated by crude extracts of CHO cells. Variant cell lines lacking adenosine kinase (EC 2.7.1.20) or deoxycytidine kinase (EC 2.7.1.74) show no resistance to tiazofurin, suggesting that these enzymes are not responsible for the phosphorylation of tiazofurin. The inhibitory activity of this agent can be reversed by exogenously supplied guanine, suggesting that the inhibition is due, at least in part, to guanylate deficiency.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
534-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Action of 2-beta-d-Ribofuranosylthiazole-4-carboxamide (tiazofurin) in Chinese hamster ovary and variant cell lines.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.