Linked Life Data

6186080

Source:http://linkedlifedata.com/resource/pubmed/id/6186080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-3-24
pubmed:abstractText
Rat 3Y1 cells were infected by AKR virus through microinjection of molecularly cloned proviral DNA. Based on a strong immunofluorescence using anti-p30 as an antiserum a cell clone (RESA-2) was selected that had a high expression of viral antigens. Subsequent restriction analysis of its DNA revealed that the RESA-2 clone contained at least 30 apparently intact integrated proviruses per genome. There was an apparently normal synthesis and processing of gag and pol gene products. The viral envelope precursor polyprotein gPr82env, however, did not yield the major envelope glycoprotein gp70. The gag precursor polyprotein, Pr65gag, as well as the gPr82env from RESA-2 cells were identified as AKR viral proteins by gel electrophoresis of hydroxylamine cleavage fragments. The virions formed by RESA-2 cells lacked gp70 and were noninfectious. After fusion of RESA-2 cells and mouse cells an infectious N-tropic virus was produced. The results indicate that rat 3Y1 cells lack (a) factor(s) necessary for the correct processing of gPr82env. The high incidence of abortive infections of murine leukemia virus (MLV) in susceptible rat cells reported by others is therefore probably due to defective particles in the virus stock and/or to the lack of (a) cellular factor(s) necessary for reverse transcription and subsequent integration of the viral genome.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
462-6
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
3Y1 rat cells are defective in processing of the envelope precursor protein of AKR virus.
pubmed:publicationType
Journal Article