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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1983-1-19
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pubmed:databankReference |
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pubmed:abstractText |
A rare cDNA coding for most of the alpha subunit of the Torpedo nicotinic acetylcholine receptor has been cloned into bacteria. The use of a mismatched oligonucleotide primer of reverse transcriptase facilitated the design of an efficient, specific probe for recombinant bacteria. DNA sequence analysis has enabled the elucidation of a large part of the polypeptide primary sequence which is discussed in relation to its acetylcholine binding activity and the location of receptor within the plasma membrane. When used as a radioactive probe, the cloned cDNA binds specifically to a single Torpedo mRNA species of about 2350 nucleotides in length but fails to show significant cross-hybridisation with alpha subunit mRNA extracted from cat muscle.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-218209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-435447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-4563441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-497150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-520545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-5963888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-6122602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-6127706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-6153123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-6159597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-6161125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-6946496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-6956875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-6997135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-7061443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-7071021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-7196502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-7240258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-7279669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-7350544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-7384786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-7394526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-881736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6183641-96437
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5809-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6183641-Amino Acid Sequence,
pubmed-meshheading:6183641-Animals,
pubmed-meshheading:6183641-Base Sequence,
pubmed-meshheading:6183641-Cell Membrane,
pubmed-meshheading:6183641-Cloning, Molecular,
pubmed-meshheading:6183641-DNA,
pubmed-meshheading:6183641-Electric Organ,
pubmed-meshheading:6183641-Nucleic Acid Hybridization,
pubmed-meshheading:6183641-Poly A,
pubmed-meshheading:6183641-RNA,
pubmed-meshheading:6183641-RNA, Messenger,
pubmed-meshheading:6183641-RNA-Directed DNA Polymerase,
pubmed-meshheading:6183641-Receptors, Cholinergic,
pubmed-meshheading:6183641-Receptors, Nicotinic,
pubmed-meshheading:6183641-Torpedo
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pubmed:year |
1982
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pubmed:articleTitle |
The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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