Linked Life Data

6183429

Source:http://linkedlifedata.com/resource/pubmed/id/6183429

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1983-1-7
pubmed:abstractText
Results are described that demonstrate that the mechanism of action of the potent cytotoxic agent 5-nitro-2'-deoxyuridine (NO2-dUrd) involves thymidine (dThd) kinase catalyzed formation of 5-nitro-2'-deoxyuridylate (NO2dUMP) and subsequent potent inhibition of thymidylate (dTMP) synthetase by this compound. The evidence for this is as follows: (a) cells lacking dThd kinase are not inhibited by high concentrations of NO2dUrd; (b) the drug has no effect on dThd or uridine (Urd) incorporation into nucleic acids but prevents incorporation of deoxyuridine (dUrd); (c) growth inhibition is reversed by dThd but not by dUrd; (d) NO2dUrd causes changes in deoxynucleoside triphosphate pool sizes which are characteristic of specific inhibition of dTMP synthetase; (e) cells treated with [3H]NO2dUrd possess macromolecular bound [3H]NO2dUMP, which has properties characteristic of the NO2dUMP-dTMP synthetase complex. Treatment of L1210 leukemic mice at 400 mg/kg daily for 6 days gave only a 33% increase in life span, probably because of its rapid degradation to the inactive nitrouracil.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-2623
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1252-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Mechanism of action of 5-nitro-2'-deoxyuridine.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.