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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1982-10-21
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pubmed:abstractText |
Fluorescein conjugated Vicia villosa (Vv) lectin was used for direct staining of the surface of viable cells of various mouse lymphocyte populations. A varying proportion of polyclonally-activated T cells expressed Vv receptor although less strongly than cells of an influenza virus-specific cytotoxic T lymphocyte (CTL) clone and blasts generated in the mixed lymphocyte reaction. Cyclosporin A (CyA) treatment markedly reduced the expression of Vv receptor following concanavalin A (Con A) stimulation by between 66% and 93% with a concurrent inhibition of blastogenesis and complete abrogation of cytolytic function. Resting mouse lymphocytes and B-cell blasts were always Vv-negative. However, non-specific suppressor factor producing non-cytotoxic T-cell lines also expressed Vv receptor as shown by the weak, but specific, surface fluorescence of EL4 and BW5147 cells stained with Vv. Vv-positive cells were not restricted to a particular Ly phenotype, Vv-positive cells being found among both Lyt 1+ and Lyt2+ MLC stimulated lymphocytes. Our data suggest that the receptor for Vv lectin cannot be regarded as an exclusive differential marker for CTL.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-1086241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-159864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-161527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-306416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-310865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-315318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-4151339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-42615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-4604193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-4609299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-6176850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-6270007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-6450806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-6451646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-6460356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-69515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-6968871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-6970130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6179856-7193084
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
769-76
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6179856-Animals,
pubmed-meshheading:6179856-Cell Line,
pubmed-meshheading:6179856-Concanavalin A,
pubmed-meshheading:6179856-Cyclosporins,
pubmed-meshheading:6179856-Fluorescein-5-isothiocyanate,
pubmed-meshheading:6179856-Fluoresceins,
pubmed-meshheading:6179856-Lectins,
pubmed-meshheading:6179856-Lymphocyte Activation,
pubmed-meshheading:6179856-Mice,
pubmed-meshheading:6179856-Mice, Inbred BALB C,
pubmed-meshheading:6179856-Mice, Inbred CBA,
pubmed-meshheading:6179856-Receptors, Mitogen,
pubmed-meshheading:6179856-Staining and Labeling,
pubmed-meshheading:6179856-T-Lymphocytes,
pubmed-meshheading:6179856-Thiocyanates
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pubmed:year |
1982
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pubmed:articleTitle |
Direct staining of mouse T lymphoblasts with fluoresceinated Vicia villosa lectin.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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